Hotspot

clpP

Genome · 75944-77993 · 2050 bp

Back to Pyracantha

Pi 0

MarkerSeek score 58.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 2050
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCTCAAATCACCAATTCGTCTGA	ACGCTTTCACATCAAAGTGA	39.1	40.0	58.1	55.9	2137-2150 bp	1.0	40.7
clpP_p2	CTCAAATCACCAATTCGTCTGA	ACGCTTTCACATCAAAGTGA	40.9	40.0	56.7	55.9	2136-2149 bp	1.0	40.7
clpP_p3	TCTCAAATCACCAATTCGTCTG	ACGCTTTCACATCAAAGTGA	40.9	40.0	56.7	55.9	2137-2150 bp	1.0	40.7
clpP_p4	TCTCAAATCACCAATTCGTCTGA	TCCATTGTTACGCTTTCACA	39.1	40.0	58.1	55.6	2146-2159 bp	1.0	40.7
clpP_p5	TCTCAAATCACCAATTCGTCTGA	CCATTGTTACGCTTTCACATCA	39.1	40.9	58.1	57.6	2145-2158 bp	1.0	40.7