Hotspot

rbcL-psaI

Genome · 62156-65086 · 2931 bp

Back to Pyracantha

Pi 0.000342

MarkerSeek score 56.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: Genome
length_bp: 2931
variable_sites: 1
pi: 0.000342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AGATCAAATTCGAATTCCAAGCA	AATGGAGGGGAGACTGTTGA	34.8	50.0	57.1	58.0	2999 bp	0.5	28.4
rbcL-psaI_p2	AGATCAAATTCGAATTCCAAGCA	GAGGGGAGACTGTTGAGAGT	34.8	55.0	57.1	58.1	2995 bp	0.5	28.2
rbcL-psaI_p3	GAGATCAAATTCGAATTCCAAGCA	AATGGAGGGGAGACTGTTGA	37.5	50.0	58.1	58.0	3000 bp	0.5	26.7
rbcL-psaI_p4	GAGATCAAATTCGAATTCCAAGCA	GAGGGGAGACTGTTGAGAGT	37.5	55.0	58.1	58.1	2996 bp	0.5	26.5
rbcL-psaI_p5	TCAAATTCGAATTCCAAGCA	AATGGAGGGGAGACTGTTGA	35.0	50.0	53.8	58.0	2996 bp	0.5	25.9