Hotspot

psbM-trnD-GUC

Genome · 33709-34932 · 1224 bp

Back to Pyracantha

Pi 0

MarkerSeek score 58.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: Genome
length_bp: 1224
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1387-1389 bp	1.0	72.8
psbM-trnD-GUC_p2	AATCACCTTGGCTGACTGTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	1389-1391 bp	1.0	71.0
psbM-trnD-GUC_p3	ATCACCTTGGCTGACTGTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	1388-1390 bp	1.0	71.0
psbM-trnD-GUC_p4	TCACCTTGGCTGACTGTTTT	GTTCAATTGGTCAGAGCACC	45.0	50.0	57.6	57.0	1388-1390 bp	1.0	68.7
psbM-trnD-GUC_p5	ATCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	42.9	50.0	58.1	58.5	1388-1390 bp	1.0	67.9