Hotspot

atpH-atpI

Genome · 16669-17829 · 1161 bp

Back to Pyracantha

Pi 0.000864

MarkerSeek score 59.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 1161
variable_sites: 1
pi: 0.000864
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1250-1253 bp	1.0	79.8
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1216-1219 bp	1.0	79.7
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	50.0	45.0	58.0	57.9	1280-1283 bp	1.0	78.9
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	45.0	45.0	58.0	57.9	1246-1249 bp	1.0	78.9
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.2	58.0	1217-1220 bp	1.0	78.6