Hotspot

trnK-UUU-trnQ-UUG

Genome · 4452-7229 · 2778 bp

Back to Pyracantha

Pi 0.00072

MarkerSeek score 56.50

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 2778
variable_sites: 2
pi: 0.00072
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2836-2837 bp	1.0	78.1
trnK-UUU-trnQ-UUG_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2835-2836 bp	1.0	78.1
trnK-UUU-trnQ-UUG_p3	CCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.3	58.1	2845-2846 bp	1.0	77.2
trnK-UUU-trnQ-UUG_p4	AAAGCCGAGTACTCTACCGT	GTGTCGACTAGAAATGGGGC	50.0	55.0	58.2	58.3	2898-2899 bp	1.0	76.0
trnK-UUU-trnQ-UUG_p5	AAGCCGAGTACTCTACCGTT	GTGTCGACTAGAAATGGGGC	50.0	55.0	58.2	58.3	2897-2898 bp	1.0	76.0