Hotspot

ycf1

SSC · 128460-134057 · 5598 bp

Pi 0.00217

MarkerSeek score 45.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5598
variable_sites: 45
pi: 0.00217
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAAATTGATCCTGTGAATGGT	TCTGATAGAACAATCACAATCAG	40.9	34.8	56.7	54.2	235 bp	1.0	41.7
ycf1_p2	TGGAAATTGATCCTGTGAATGGT	TCTGATAGAACAATCACAATCAG	39.1	34.8	58.3	54.2	236 bp	0.9	37.0
ycf1_p3	TGGAAATTGATCCTGTGAATGG	TCTGATAGAACAATCACAATCAG	40.9	34.8	56.7	54.2	236 bp	0.9	37.0
ycf1_p4	TGGAAATTGATCCTGTGAATGGT	AGATCTTTCTGATAGAACAATCACA	39.1	32.0	58.3	56.1	243 bp	0.9	37.0
ycf1_p5	TGGAAATTGATCCTGTGAATGG	AGATCTTTCTGATAGAACAATCACA	40.9	32.0	56.7	56.1	243 bp	0.9	37.0