Hotspot

ndhH-rps15

SSC · 127696-127793 · 98 bp

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Pi 0.0154
MarkerSeek score 50.10
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
98
variable_sites
3
pi
0.0154
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 AACACCATGCATTGATGGGT CAGTTGGATATTCGGGAGCC 45.0 55.0 58.1 58.1 206 bp 1.0 79.8
ndhH-rps15_p2 AACACCATGCATTGATGGGT TCAACGGTTGCTGGCTTATT 45.0 45.0 58.1 58.1 267 bp 1.0 79.7
ndhH-rps15_p3 ATGCATTGATGGGTGGTGAG CAGTTGGATATTCGGGAGCC 50.0 55.0 58.2 58.1 200 bp 1.0 78.4
ndhH-rps15_p4 AACACCATGCATTGATGGGT CGGTTGCTGGCTTATTTGTC 45.0 50.0 58.1 57.7 263 bp 1.0 78.3
ndhH-rps15_p5 ATGCATTGATGGGTGGTGAG TCAACGGTTGCTGGCTTATT 50.0 45.0 58.2 58.1 261 bp 1.0 78.3