Hotspot

rps3-rpl22

LSC · 87151-87185 · 35 bp

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Pi 0.0588
MarkerSeek score 64.50
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
35
variable_sites
5
pi
0.0588
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 CGTGCAATTCCTTCGAAACC AAAGCCGAAGCCAATAGGAG 50.0 50.0 58.0 58.0 365-367 bp 1.0 81.1
rps3-rpl22_p2 CGTGCAATTCCTTCGAAACC TCTGACTTTCGCGAGACATG 50.0 50.0 58.0 58.0 773-775 bp 1.0 80.7
rps3-rpl22_p3 CGTGCAATTCCTTCGAAACC GTGAATCAAACAAAGGCGGG 50.0 50.0 58.0 57.9 672-674 bp 1.0 80.4
rps3-rpl22_p4 CGTGCAATTCCTTCGAAACC TAGTAACTTGGTCTCGGGCA 50.0 50.0 58.0 58.1 912-914 bp 1.0 80.1
rps3-rpl22_p5 TTCTTCGATTCCTCGCGTTT TCTGACTTTCGCGAGACATG 45.0 50.0 57.9 58.0 870-872 bp 0.6 63.7