Hotspot

accD-psaI

LSC · 61263-62206 · 944 bp

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Pi 0.00389
MarkerSeek score 55.00
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
944
variable_sites
11
pi
0.00389
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGCTCCATGGTTTCTTTCCC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.8 58.0 1055-1068 bp 1.0 79.4
accD-psaI_p2 GCTCCATGGTTTCTTTCCCT CCATTGCAATTGCCGGAAAT 50.0 45.0 57.8 58.0 1054-1067 bp 1.0 79.4
accD-psaI_p3 AGCTCCATGGTTTCTTTCCC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.8 57.9 1061-1074 bp 1.0 78.7
accD-psaI_p4 GCTCCATGGTTTCTTTCCCT AAGAAGCCATTGCAATTGCC 50.0 45.0 57.8 57.9 1060-1073 bp 1.0 78.7
accD-psaI_p5 GACCCAATCGTACCACGTAA CCATTGCAATTGCCGGAAAT 50.0 45.0 57.3 58.0 1107-1120 bp 1.0 75.9