Hotspot

atpB-rbcL

LSC · 56826-57626 · 801 bp

Back to Puya

Pi 0.00433
MarkerSeek score 55.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
801
variable_sites
9
pi
0.00433
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GGGGAAAAACGACATCCAGT ACACCAGCTTTAAATCCAGCA 50.0 42.9 58.1 58.1 956-973 bp 1.0 71.3
atpB-rbcL_p2 GGGGAAAAACGACATCCAGT ACCAGCTTTAAATCCAGCACT 50.0 42.9 58.1 57.8 954-971 bp 1.0 71.0
atpB-rbcL_p3 TGGGCCAATAATTTGAGCGA ACACCAGCTTTAAATCCAGCA 45.0 42.9 57.9 58.1 934-951 bp 1.0 71.0
atpB-rbcL_p4 TGAGCGATACACCCTAGGTT ACACCAGCTTTAAATCCAGCA 50.0 42.9 57.8 58.1 921-938 bp 1.0 70.9
atpB-rbcL_p5 TTGAGCGATACACCCTAGGT ACACCAGCTTTAAATCCAGCA 50.0 42.9 57.8 58.1 922-939 bp 1.0 70.9