Hotspot

psbM-trnD-GUC

LSC · 31796-32840 · 1045 bp

Back to Puya

Pi 0.00325
MarkerSeek score 55.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1045
variable_sites
11
pi
0.00325
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC CCTCGCTGGGATTGTAGTTC 45.0 55.0 55.8 58.1 1164-1173 bp 1.0 63.5
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC TACACCTCGCTGGGATTGTA 45.0 50.0 55.8 58.1 1168-1177 bp 1.0 62.9
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC CATACACCTCGCTGGGATTG 45.0 55.0 55.8 58.4 1170-1179 bp 1.0 60.7
psbM-trnD-GUC_p4 AGGAACTAGAATGAACAGTGCA CCTCGCTGGGATTGTAGTTC 40.9 55.0 57.4 58.1 1171-1180 bp 1.0 60.4
psbM-trnD-GUC_p5 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 1147-1156 bp 1.0 60.2