Hotspot

trnC-GCA-petN

LSC · 29360-30387 · 1028 bp

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Pi 0.0021
MarkerSeek score 53.30
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1028
variable_sites
7
pi
0.0021
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 AGTTCAAATCTGGGTGTCGC AGTCCACTCCTTCCCCATAC 50.0 55.0 58.5 58.1 1135-1139 bp 1.0 75.9
trnC-GCA-petN_p2 AGTTCAAATCTGGGTGTCGC GTCCACTCCTTCCCCATACT 50.0 55.0 58.5 58.1 1134-1138 bp 1.0 75.9
trnC-GCA-petN_p3 AGTTCAAATCTGGGTGTCGC AGCCCAAGCGAGACTTACTA 50.0 50.0 58.5 58.2 1076-1080 bp 1.0 75.6
trnC-GCA-petN_p4 AGTTCAAATCTGGGTGTCGC GAGTCCACTCCTTCCCCATA 50.0 55.0 58.5 57.8 1136-1140 bp 1.0 75.4
trnC-GCA-petN_p5 AGTTCAAATCTGGGTGTCGC CTAGAGTCCACTCCTTCCCC 50.0 60.0 58.5 58.2 1139-1143 bp 1.0 75.1