Hotspot

psaJ-rpl33

LSC · 70223-70748 · 526 bp

Back to Purshia

Pi 0

MarkerSeek score 61.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 526
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTGGCACC	AACATCTTTACCCTTGGCCA	50.0	45.0	58.0	57.0	648-667 bp	1.0	74.4
psaJ-rpl33_p2	TCCGGATGCGTTGATATTCC	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	565-584 bp	1.0	73.8
psaJ-rpl33_p3	GTTCGGGTCTTTAGCAGGTT	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	610-629 bp	1.0	73.5
psaJ-rpl33_p4	ACATATCTTTCCGTGGCACC	CACGATTTCGAACACAACTGG	50.0	47.6	58.0	58.1	694-713 bp	1.0	73.4
psaJ-rpl33_p5	TGGCACCGGTAGTAAGTACT	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	635-654 bp	1.0	73.0