Hotspot

trnS-UGA-psbZ

LSC · 37440-37821 · 382 bp

Back to Purshia

Pi 0.0132

MarkerSeek score 62.80

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 5
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	AAGGAGAGAGAGGGATTCGA	CAACCCTCAGGAGAAGCAAA	50.0	50.0	56.9	57.7	570-574 bp	1.0	70.0
trnS-UGA-psbZ_p2	AGGAGAGAGAGGGATTCGAA	CAACCCTCAGGAGAAGCAAA	50.0	50.0	56.9	57.7	569-573 bp	1.0	70.0
trnS-UGA-psbZ_p3	AAGGAGAGAGAGGGATTCGA	TTTTGTTACCCGACCAACCC	50.0	50.0	56.9	58.3	584-588 bp	1.0	69.7
trnS-UGA-psbZ_p4	AGGAGAGAGAGGGATTCGAA	TTTTGTTACCCGACCAACCC	50.0	50.0	56.9	58.3	583-587 bp	1.0	69.7
trnS-UGA-psbZ_p5	GAGAGAGAGGGATTCGAACC	CAACCCTCAGGAGAAGCAAA	55.0	50.0	56.8	57.7	567-571 bp	1.0	69.7