Hotspot

trnT-GGU-psbD

LSC · 33400-34689 · 1290 bp

Back to Purshia

Pi 0.00949
MarkerSeek score 66.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1290
variable_sites
12
pi
0.00949
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1454-1465 bp 1.0 81.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1457-1468 bp 1.0 81.3
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1456-1467 bp 1.0 81.3
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 1459-1470 bp 1.0 81.1
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGCCAGACCAACCTACAAAA 50.0 50.0 58.3 58.0 1431-1442 bp 1.0 79.8