Hotspot

petN-psbM

LSC · 29971-30810 · 840 bp

Back to Purshia

Pi 0.00476

MarkerSeek score 61.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 4
pi: 0.00476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	AAAACAGTCAGCCAAGGTGA	45.0	45.0	58.1	57.6	909 bp	1.0	76.0
petN-psbM_p2	CCCTTTCGCTCGTAGTATGG	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.9	57.6	902 bp	1.0	75.8
petN-psbM_p3	CCTTTCGCTCGTAGTATGGG	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.9	57.6	901 bp	1.0	75.8
petN-psbM_p4	CTTTCGCTCGTAGTATGGGG	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.9	57.6	900 bp	1.0	75.8
petN-psbM_p5	TTCGCTCGTAGTATGGGGAA	AAAACAGTCAGCCAAGGTGA	50.0	45.0	58.2	57.6	898 bp	1.0	75.0