Hotspot

psbK-psbI

LSC · 8022-8278 · 257 bp

Back to Purshia

Pi 0.012

MarkerSeek score 62.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 3
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	388-394 bp	1.0	74.3
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	385-391 bp	1.0	72.3
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	386-392 bp	1.0	72.3
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	387-393 bp	1.0	72.3
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCAGGATTACG	50.0	47.6	57.9	57.8	389-395 bp	1.0	72.0