Hotspot

trnH-GUG-psbA

LSC · 75-459 · 385 bp

Back to Purshia

Pi 0.0341
MarkerSeek score 73.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
385
variable_sites
13
pi
0.0341
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 448-451 bp 1.0 81.5
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 498-501 bp 1.0 81.4
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 512-515 bp 1.0 81.3
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 511-514 bp 1.0 81.3
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 513-516 bp 1.0 81.3