Hotspot

rps16

LSC · 1319-2436 · 1118 bp

Back to Pulsatilla

Pi 0.00556

MarkerSeek score 55.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1118
variable_sites: 19
pi: 0.00556
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CCCATACCGGTTCCAAACAA	AATTTGATTTCGCGTAACGT	50.0	35.0	58.1	54.6	1187-1194 bp	1.0	54.8
rps16_p2	TCAATCCCATACCGGTTCCA	AATTTGATTTCGCGTAACGT	50.0	35.0	58.4	54.6	1192-1199 bp	1.0	52.2
rps16_p3	CAATCCCATACCGGTTCCAA	AATTTGATTTCGCGTAACGT	50.0	35.0	57.6	54.6	1191-1198 bp	1.0	52.0
rps16_p4	ATCCCATACCGGTTCCAAAC	AATTTGATTTCGCGTAACGT	50.0	35.0	57.6	54.6	1189-1196 bp	1.0	52.0
rps16_p5	CCCATACCGGTTCCAAACAA	TTTGATTTCGCGTAACGTAA	50.0	35.0	58.1	54.0	1186-1192 bp	0.75	39.6