Hotspot

ndhH-rps15

SSC · 126148-126343 · 196 bp

Back to Pulsatilla

Pi 0.109

MarkerSeek score 72.60

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 196
variable_sites: 47
pi: 0.109
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	ATGAGTCGAAGAACACCGTG	AACGGCTGCTGGCTTATTTA	50.0	45.0	57.9	57.9	319-471 bp	1.0	90.5
ndhH-rps15_p2	GCATTGATGGGTGTTGAGGA	AACGGCTGCTGGCTTATTTA	50.0	45.0	58.2	57.9	300-452 bp	1.0	89.7
ndhH-rps15_p3	ATGAGTCGAAGAACACCGTG	ACGGCTGCTGGCTTATTTAT	50.0	45.0	57.9	57.7	318-470 bp	1.0	88.7
ndhH-rps15_p4	TGATGAGTCGAAGAACACCG	AACGGCTGCTGGCTTATTTA	50.0	45.0	57.7	57.9	321-473 bp	1.0	88.2
ndhH-rps15_p5	GATGAGTCGAAGAACACCGT	AACGGCTGCTGGCTTATTTA	50.0	45.0	57.7	57.9	320-472 bp	1.0	88.2