Hotspot

ndhF-trnL-UAG

SSC · 115760-117037 · 1278 bp

Back to Pulsatilla

Pi 0.0649

MarkerSeek score 85.40

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1278
variable_sites: 256
pi: 0.0649
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	ACGAAAGGTATGATCCACGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1348-2329 bp	1.0	91.4
ndhF-trnL-UAG_p2	CGAAAGGTATGATCCACGCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1347-2328 bp	1.0	91.4
ndhF-trnL-UAG_p3	ACGAAAGGTATGATCCACGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1349-2330 bp	1.0	91.4
ndhF-trnL-UAG_p4	CGAAAGGTATGATCCACGCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1348-2329 bp	1.0	91.4
ndhF-trnL-UAG_p5	ACGAAAGGTATGATCCACGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1358-2339 bp	1.0	90.5