Hotspot

trnN-GUU-ndhF

IRb · 111297-113527 · 2231 bp

Back to Pulsatilla

Pi 0.0285

MarkerSeek score 68.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2231
variable_sites: 130
pi: 0.0285
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	TCAAAGCGAGCCCTTTCATT	CAGGTATAAGAAGGCTGGCG	45.0	55.0	58.1	58.1	2488-2994 bp	1.0	86.4
trnN-GUU-ndhF_p2	GGAACGGGTTACCTACTCCT	CAGGTATAAGAAGGCTGGCG	55.0	55.0	58.1	58.1	2513-2863 bp	0.875	80.9
trnN-GUU-ndhF_p3	TTGTGTTTTACTCCCCGCAA	CAGGTATAAGAAGGCTGGCG	45.0	55.0	57.9	58.1	2846-2952 bp	0.75	74.3
trnN-GUU-ndhF_p4	TTTGTGTTTTACTCCCCGCA	CAGGTATAAGAAGGCTGGCG	45.0	55.0	57.9	58.1	2847-2953 bp	0.75	74.3
trnN-GUU-ndhF_p5	TGTTTTACTCCCCGCAACTC	CAGGTATAAGAAGGCTGGCG	50.0	55.0	58.1	58.1	2843-2949 bp	0.75	73.8