Hotspot

petA-psbJ

LSC · 65118-65678 · 561 bp

Back to Pulsatilla

Pi 0.0509

MarkerSeek score 84.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 49
pi: 0.0509
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCATCTGTTATTCTGGCA	TGGCTTATAGGCACTGTAGC	45.0	50.0	57.2	57.1	598-740 bp	1.0	76.3
petA-psbJ_p2	TGGCATCTGTTATTCTGGCA	AGGCACTGTAGCTGGTATTC	45.0	50.0	57.2	57.0	590-732 bp	1.0	75.7
petA-psbJ_p3	TGGCATCTGTTATTCTGGCA	GGCACTGTAGCTGGTATTCT	45.0	50.0	57.2	57.0	589-731 bp	1.0	75.7
petA-psbJ_p4	TGGCATCTGTTATTCTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.2	58.1	580-722 bp	1.0	75.0
petA-psbJ_p5	TGGCATCTGTTATTCTGGCA	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.2	57.8	581-723 bp	1.0	74.2