Hotspot

trnM-CAU-atpE

LSC · 54180-54447 · 268 bp

Back to Pulsatilla

Pi 0.0204

MarkerSeek score 61.80

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 268
variable_sites: 13
pi: 0.0204
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	TAAGTAGCGCTGAAGGCAAG	50.0	50.0	58.1	58.0	398-459 bp	1.0	84.1
trnM-CAU-atpE_p2	TTGCTTTCATACGGCAGGAG	TAAGTAGCGCTGAAGGCAAG	50.0	50.0	58.3	58.0	410-471 bp	1.0	82.6
trnM-CAU-atpE_p3	CATACGGCAGGAGTCATTGG	TAAGTAGCGCTGAAGGCAAG	55.0	50.0	58.4	58.0	403-464 bp	1.0	81.4
trnM-CAU-atpE_p4	ATTGCTTTCATACGGCAGGA	TAAGTAGCGCTGAAGGCAAG	45.0	50.0	57.6	58.0	411-472 bp	1.0	81.3
trnM-CAU-atpE_p5	CCCAGCGGTTAGAGTATTGC	TAAGTAGCGCTGAAGGCAAG	55.0	50.0	58.4	58.0	426-487 bp	1.0	81.2