Hotspot

ndhC-trnV-UAC

LSC · 52133-53255 · 1123 bp

Back to Pulsatilla

Pi 0.035
MarkerSeek score 74.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1123
variable_sites
84
pi
0.035
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.89
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCTGGCCCTTCGTTAATTGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1238-1322 bp 1.0 86.7
ndhC-trnV-UAC_p2 CCTTCGTTAATTGGGGCCAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1232-1316 bp 1.0 84.1
ndhC-trnV-UAC_p3 TAATTGGGGCCAAAACTCCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1225-1309 bp 1.0 84.1
ndhC-trnV-UAC_p4 TCTGGCCCTTCGTTAATTGG GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1237-1321 bp 1.0 83.9
ndhC-trnV-UAC_p5 TCTGGCCCTTCGTTAATTGG GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1226-1310 bp 1.0 82.8