Hotspot

psbD-trnT-GGU

LSC · 21793-23092 · 1300 bp

Back to Pulsatilla

Pi 0.0111

MarkerSeek score 57.80

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1300
variable_sites: 35
pi: 0.0111
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TCTCCGTAACCAGTCATCCA	GCCCTTTTAACTCAGCGGTA	50.0	50.0	57.8	57.9	1431-1575 bp	1.0	82.3
psbD-trnT-GGU_p2	TCTCCGTAACCAGTCATCCA	ATGGGCCCTTTTAACTCAGC	50.0	50.0	57.8	58.1	1435-1579 bp	1.0	81.9
psbD-trnT-GGU_p3	TCTCCGTAACCAGTCATCCA	GGCGTAAGTCATCGGTTCAA	50.0	50.0	57.8	58.3	1394-1538 bp	1.0	81.0
psbD-trnT-GGU_p4	GGTCTCTCCGTAACCAGTCA	GCCCTTTTAACTCAGCGGTA	55.0	50.0	58.5	57.9	1435-1579 bp	1.0	80.4
psbD-trnT-GGU_p5	GGTCTCTCCGTAACCAGTCA	ATGGGCCCTTTTAACTCAGC	55.0	50.0	58.5	58.1	1439-1583 bp	1.0	80.0