Hotspot

psbZ-trnS-UGA

LSC · 18705-19078 · 374 bp

Back to Pulsatilla

Pi 0.0118

MarkerSeek score 58.80

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 12
pi: 0.0118
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	CCAACCGTCAGAAGAAGCAA	CAACCACTCAGCCATCTCTC	50.0	55.0	58.4	58.0	470-494 bp	1.0	82.6
psbZ-trnS-UGA_p2	CCAACCGTCAGAAGAAGCAA	GAGCTATCAACCACTCAGCC	50.0	55.0	58.4	58.1	477-501 bp	1.0	82.3
psbZ-trnS-UGA_p3	CCAACCGTCAGAAGAAGCAA	GGAGCTATCAACCACTCAGC	50.0	55.0	58.4	58.1	478-502 bp	1.0	82.3
psbZ-trnS-UGA_p4	CCAACCGTCAGAAGAAGCAA	CAAGACCGGAGCTATCAACC	50.0	55.0	58.4	58.1	485-509 bp	1.0	82.2
psbZ-trnS-UGA_p5	TGACCAACCGTCAGAAGAAG	CAACCACTCAGCCATCTCTC	50.0	55.0	57.5	58.0	473-497 bp	1.0	81.6