Hotspot

rps16-trnK-UUU

LSC · 2437-3274 · 838 bp

Back to Pulsatilla

Pi 0.03

MarkerSeek score 64.20

Primer available yes

Label rps16-trnK-UUU

Feature Metadata

label_name: rps16-trnK-UUU
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 56
pi: 0.03
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnK-UUU_p1	GCTCAACCTACGGAAACTGT	AAAGCCGAGTACTCTACCGT	50.0	50.0	57.8	58.2	916-1096 bp	1.0	85.8
rps16-trnK-UUU_p2	GCTCAACCTACGGAAACTGT	AAGCCGAGTACTCTACCGTT	50.0	50.0	57.8	58.2	915-1095 bp	1.0	85.8
rps16-trnK-UUU_p3	GCTCAACCTACGGAAACTGT	CCGCACTTAAAAGCCGAGTA	50.0	50.0	57.8	58.3	925-1105 bp	1.0	84.9
rps16-trnK-UUU_p4	GCTCAACCTACGGAAACTGT	TACCGTTGAGTTAGCAACCC	50.0	50.0	57.8	57.5	902-1082 bp	1.0	83.6
rps16-trnK-UUU_p5	GCTCAACCTACGGAAACTGT	CGAATCCGTTGCTTCATCCA	50.0	50.0	57.8	58.6	964-1144 bp	1.0	81.9