Hotspot

ycf1

SSC · 119674-125043 · 5370 bp

Back to Pulmonaria

Pi 0.000559

MarkerSeek score 45.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5370
variable_sites: 3
pi: 0.000559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAATTGTGTTTTTCCTTTTAAA	TCCATCTGAAACCTTGGCAC	26.1	50.0	52.6	58.1	243 bp	1.0	40.7
ycf1_p2	GGAATTGTGTTTTTCCTTTTAAAA	TCCATCTGAAACCTTGGCAC	25.0	50.0	53.4	58.1	243 bp	1.0	40.7
ycf1_p3	GGAATTGTGTTTTTCCTTTTAAA	CCATCTGAAACCTTGGCACA	26.1	50.0	52.6	58.4	242 bp	1.0	40.7
ycf1_p4	GGAATTGTGTTTTTCCTTTTAAAA	CCATCTGAAACCTTGGCACA	25.0	50.0	53.4	58.4	242 bp	1.0	40.7
ycf1_p5	ACTTTTATAAGGAATTGTGTTTTTCC	TCCATCTGAAACCTTGGCAC	26.9	50.0	55.2	58.1	253 bp	1.0	40.6