Hotspot

rps16

LSC · 4824-5953 · 1130 bp

Back to Pulmonaria

Pi 0.000885

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1130
variable_sites: 1
pi: 0.000885
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGGATAGAGCAGAAAGGGGG	TCAACTACGCACCCCTTCTA	55.0	50.0	58.5	58.1	1239 bp	1.0	75.6
rps16_p2	GGGTGTCTACGGATTGGGTA	TCAACTACGCACCCCTTCTA	55.0	50.0	58.5	58.1	1284 bp	1.0	75.4
rps16_p3	TGGATAGAGCAGAAAGGGGG	AATCAACTACGCACCCCTTC	55.0	50.0	58.5	57.9	1241 bp	1.0	75.3
rps16_p4	TGGATAGAGCAGAAAGGGGG	GAATCAACTACGCACCCCTT	55.0	50.0	58.5	57.9	1242 bp	1.0	75.3
rps16_p5	GGGTGTCTACGGATTGGGTA	AATCAACTACGCACCCCTTC	55.0	50.0	58.5	57.9	1286 bp	1.0	75.1