Hotspot

petD

LSC · 73471-74702 · 1232 bp

Back to Pulmonaria

Pi 0.00244

MarkerSeek score 60.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1232
variable_sites: 3
pi: 0.00244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTTCCCTTCAACTCGCCTC	TCCCTAGATACACACGTCGT	50.0	50.0	57.9	57.6	1344 bp	1.0	76.0
petD_p2	CCCTTCAACTCGCCTCTTTA	TCCCTAGATACACACGTCGT	50.0	50.0	57.2	57.6	1340 bp	1.0	70.8
petD_p3	TCCCTTCAACTCGCCTCTTT	TCCCTAGATACACACGTCGT	50.0	50.0	58.9	57.6	1341 bp	1.0	69.4
petD_p4	TTCCCTTCAACTCGCCTCTT	TCCCTAGATACACACGTCGT	50.0	50.0	58.9	57.6	1342 bp	1.0	69.4
petD_p5	TTTCCCTTCAACTCGCCTCT	TCCCTAGATACACACGTCGT	50.0	50.0	58.9	57.6	1343 bp	1.0	69.4