Hotspot

rps12-rps7

IRa · 136323-136375 · 53 bp

Back to Pulmonaria

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRa
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	GGCTTGGAGGGAGATCTTTC	TTTTTCTGCAGTACCTCGGC	55.0	50.0	57.7	58.5	149 bp	1.0	74.0
rps12-rps7_p2	TATGTACGGCTTGGAGGGAG	TTTTTCTGCAGTACCTCGGC	55.0	50.0	58.6	58.5	156 bp	1.0	71.9
rps12-rps7_p3	GTATGTACGGCTTGGAGGGA	TTTTTCTGCAGTACCTCGGC	55.0	50.0	58.9	58.5	157 bp	1.0	69.6
rps12-rps7_p4	GCCGTATTCGATGAAAGTCG	TTTTTCTGCAGTACCTCGGC	50.0	50.0	57.1	58.5	176 bp	1.0	69.4
rps12-rps7_p5	GGCTTGGAGGGAGATCTTTC	TTTTCTGCAGTACCTCGGC	55.0	52.6	57.7	57.8	148 bp	1.0	68.0