Hotspot

rpl32-trnL-UAG

SSC · 109614-110093 · 480 bp

Back to Pulmonaria

Pi 0.00208
MarkerSeek score 55.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
480
variable_sites
1
pi
0.00208
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAAGGGGTATTGGACAGCAT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.3 57.9 616 bp 1.0 73.2
rpl32-trnL-UAG_p2 GAAGGGGTATTGGACAGCAT AGCAGCGTGTCTACCAATTT 50.0 45.0 57.3 57.8 609 bp 1.0 72.8
rpl32-trnL-UAG_p3 GAAGGGGTATTGGACAGCAT CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.3 57.8 615 bp 1.0 72.7
rpl32-trnL-UAG_p4 GAAGGGGTATTGGACAGCAT CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.3 57.8 614 bp 1.0 72.7
rpl32-trnL-UAG_p5 GAAGGGGTATTGGACAGCAT GAGCAGCGTGTCTACCAATT 50.0 50.0 57.3 58.3 610 bp 1.0 72.1