Hotspot

ndhF-rpl32

SSC · 108859-109451 · 593 bp

Back to Pulmonaria

Pi 0.00337
MarkerSeek score 60.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
593
variable_sites
2
pi
0.00337
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGCAAAGGTATGATCCACGC ATGCTGTCCAATACCCCTTC 50.0 50.0 58.3 57.3 719 bp 1.0 71.7
ndhF-rpl32_p2 AGCAAAGGTATGATCCACGC AATGCTGTCCAATACCCCTTC 50.0 47.6 58.3 58.0 720 bp 1.0 69.4
ndhF-rpl32_p3 GCAAAGGTATGATCCACGCA ATGCTGTCCAATACCCCTTC 50.0 50.0 58.6 57.3 718 bp 1.0 69.4
ndhF-rpl32_p4 AGCAAAGGTATGATCCACGC GCTGTCCAATACCCCTTCCT 50.0 55.0 58.3 59.1 717 bp 1.0 68.9
ndhF-rpl32_p5 AGCAAAGGTATGATCCACGC AATGCTGTCCAATACCCCTT 50.0 45.0 58.3 56.8 720 bp 1.0 67.8