Hotspot

ndhC-trnV-UAC

LSC · 49539-50313 · 775 bp

Back to Pulmonaria

Pi 0.00129
MarkerSeek score 59.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
775
variable_sites
1
pi
0.00129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGGGGCTAAAACTCCTGAAA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.8 58.1 910 bp 1.0 77.7
ndhC-trnV-UAC_p2 CTCCGGCCCTTTTCTAATCG CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.4 58.1 928 bp 1.0 75.9
ndhC-trnV-UAC_p3 ATCGGGGCTAAAACTCCTGA CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.4 58.1 912 bp 1.0 75.9
ndhC-trnV-UAC_p4 AATCGGGGCTAAAACTCCTG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.6 58.1 913 bp 1.0 75.9
ndhC-trnV-UAC_p5 CGGGGCTAAAACTCCTGAAA AGTGCATGTTGGGTCTTTGA 50.0 45.0 57.8 57.6 974 bp 1.0 75.6