Hotspot

trnT-GGU-psbD

LSC · 30804-31975 · 1172 bp

Back to Pulmonaria

Pi 0.00171

MarkerSeek score 59.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1172
variable_sites: 2
pi: 0.00171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCGCAACCAGTCATCCATAA	55.0	50.0	58.1	58.0	1302 bp	1.0	79.3
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.1	58.0	1305 bp	1.0	79.1
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	CCGCAACCAGTCATCCATAA	50.0	50.0	58.1	58.0	1321 bp	1.0	79.1
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1332 bp	1.0	78.7
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1335 bp	1.0	78.5