Hotspot

atpH-atpI

LSC · 12857-13834 · 978 bp

Back to Pulmonaria

Pi 0.00409

MarkerSeek score 61.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 978
variable_sites: 4
pi: 0.00409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGCAATAACAGAAGCAGC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.1	57.9	1071 bp	1.0	78.6
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.1	57.9	1148 bp	1.0	78.2
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.9	57.9	1152 bp	1.0	78.2
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1081 bp	1.0	78.1
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	1080 bp	1.0	77.6