Hotspot

ycf1

SSC · 124252-129594 · 5343 bp

Back to Pugionium

Pi 0.00299

MarkerSeek score 43.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5343
variable_sites: 24
pi: 0.00299
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAATTCCAGGTTCCGTTGGT	AGGCGGATCTTGGATAAACA	50.0	45.0	58.1	56.6	350 bp	1.0	70.1
ycf1_p2	CCACTACGCGTTTTGCTTTT	AGGCGGATCTTGGATAAACA	45.0	45.0	57.9	56.6	377 bp	1.0	69.8
ycf1_p3	CCAGGTTCCGTTGGTACATT	AGGCGGATCTTGGATAAACA	50.0	45.0	57.8	56.6	345 bp	1.0	69.4
ycf1_p4	GAACGAATTCCAGGTTCCGT	AGGCGGATCTTGGATAAACA	50.0	45.0	58.2	56.6	354 bp	1.0	69.3
ycf1_p5	TGAACGAATTCCAGGTTCCG	AGGCGGATCTTGGATAAACA	50.0	45.0	58.2	56.6	355 bp	1.0	69.3