Hotspot

rps16

LSC · 5050-6181 · 1132 bp

Back to Pugionium

Pi 0.00177

MarkerSeek score 33.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1132
variable_sites: 3
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CATGTGTGCGTCAGGGATAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	2189 bp	1.0	79.6
rps16_p2	GATGGAAGCGAAAGAGGAGG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.1	58.1	1635-1636 bp	1.0	79.4
rps16_p3	AAATCCAGTCATGTGTGCGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	2198 bp	1.0	79.0
rps16_p4	CATGTGTGCGTCAGGGATAA	ATCCTTCCGTCCCAGAGTAC	50.0	55.0	58.0	58.2	2180 bp	1.0	78.4
rps16_p5	CATGTGTGCGTCAGGGATAA	TGACTGAACCTTTTACGCGT	50.0	45.0	58.0	57.8	1929 bp	1.0	78.4