Hotspot

rpoC2

LSC · 15876-19967 · 4092 bp

Back to Pugionium

Pi 0.00147

MarkerSeek score 54.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4092
variable_sites: 9
pi: 0.00147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CAATCGATACGACCCCGAAG	AGGTTTATCGATCCCAGGGG	55.0	55.0	58.2	58.6	304 bp	1.0	73.9
rpoC2_p2	AATCGATACGACCCCGAAGA	AGGTTTATCGATCCCAGGGG	50.0	55.0	58.3	58.6	303 bp	1.0	73.2
rpoC2_p3	CAATCGATACGACCCCGAAG	CAGGGGGTGCAGATTCATAA	55.0	50.0	58.2	57.3	290 bp	1.0	72.5
rpoC2_p4	CAACCAATCGATACGACCCC	AGGTTTATCGATCCCAGGGG	55.0	55.0	58.4	58.6	308 bp	1.0	72.4
rpoC2_p5	CAGGCCTTTCAACCAATCGA	AGGTTTATCGATCCCAGGGG	50.0	55.0	58.5	58.6	317 bp	1.0	72.0