Hotspot

rpoA

LSC · 77769-78752 · 984 bp

Back to Pugionium

Pi 0.00271

MarkerSeek score 58.10

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 984
variable_sites: 4
pi: 0.00271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGGGTTTGACTGGAAAATCGA	TAAAGGCTGAAAGGGTTTCA	42.9	40.0	57.8	54.8	1142 bp	1.0	44.6
rpoA_p2	TGGGTTTGACTGGAAAATCGA	GGTTGTAGACCCCCTAAAAA	42.9	45.0	57.8	54.5	1182 bp	1.0	42.8
rpoA_p3	TGGGTTTGACTGGAAAATCGA	ATAAAGGCTGAAAGGGTTTCA	42.9	38.1	57.8	55.4	1143 bp	1.0	41.5
rpoA_p4	TGGGTTTGACTGGAAAATCG	TAAAGGCTGAAAGGGTTTCA	45.0	40.0	56.2	54.8	1142 bp	1.0	40.3
rpoA_p5	TGGGTTTGACTGGAAAATCGA	AATAAAGGCTGAAAGGGTTTCA	42.9	36.4	57.8	56.1	1144 bp	1.0	40.3