Hotspot

rpl20

LSC · 68405-68758 · 354 bp

Back to Pugionium

Pi 0.00377

MarkerSeek score 56.00

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 354
variable_sites: 2
pi: 0.00377
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	CCCCGAGCTCATTCTCCTTA	TGACTAGTAAAGGGGAAAAGA	55.0	38.1	58.6	53.5	483 bp	1.0	40.3
rpl20_p2	CTACTCTACCTTCCCCGAGC	TGACTAGTAAAGGGGAAAAGA	60.0	38.1	58.7	53.5	495 bp	1.0	40.3
rpl20_p3	CCCCGAGCTCATTCTCCTTA	TGACTAGTAAAGGGGAAAAGAA	55.0	36.4	58.6	54.4	483 bp	1.0	40.3
rpl20_p4	CTACTCTACCTTCCCCGAGC	TGACTAGTAAAGGGGAAAAGAA	60.0	36.4	58.7	54.4	495 bp	1.0	40.3
rpl20_p5	CCCGAGCTCATTCTCCTTAG	TGACTAGTAAAGGGGAAAAGA	55.0	38.1	57.2	53.5	482 bp	1.0	40.3