Hotspot

petD

LSC · 76360-77567 · 1208 bp

Back to Pugionium

Pi 0.00166

MarkerSeek score 46.60

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1208
variable_sites: 3
pi: 0.00166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GGGTGTATTGACCGCATCTT	CTTCATGAAGCCTCCCGAAA	50.0	50.0	58.0	57.9	2219-2256 bp	1.0	79.1
petD_p2	GGGTGTATTGACCGCATCTT	TTCATGCCTGTTCAAAACGC	50.0	45.0	58.0	57.9	2336-2373 bp	1.0	78.9
petD_p3	CCGTCCAACTGTTACTGAGG	CTTCATGAAGCCTCCCGAAA	55.0	50.0	57.9	57.9	2417-2454 bp	1.0	78.7
petD_p4	GGGTGTATTGACCGCATCTT	AAGCACTTCATGAAGCCTCC	50.0	50.0	58.0	58.2	2224-2261 bp	1.0	78.7
petD_p5	CCGTCCAACTGTTACTGAGG	TTCATGCCTGTTCAAAACGC	55.0	45.0	57.9	57.9	2534-2571 bp	1.0	78.6