Hotspot

atpA

LSC · 9857-11380 · 1524 bp

Back to Pugionium

Pi 0.00131

MarkerSeek score 55.60

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 3
pi: 0.00131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AAGAATGATCCATTTCTTTTCTT	TGGTATGTTTGGTACGATGA	26.1	40.0	52.6	54.1	1652 bp	1.0	40.1
atpA_p2	AGAATGATCCATTTCTTTTCTTT	TGGTATGTTTGGTACGATGA	26.1	40.0	52.6	54.1	1651 bp	1.0	40.1
atpA_p3	AAGAATGATCCATTTCTTTTCTT	TGGTATGTTTGGTACGATGAA	26.1	38.1	52.6	55.0	1652 bp	1.0	40.1
atpA_p4	AGAATGATCCATTTCTTTTCTTT	TGGTATGTTTGGTACGATGAA	26.1	38.1	52.6	55.0	1651 bp	1.0	40.1
atpA_p5	AGAATGATCCATTTCTTTTCTTT	TTGGTATGTTTGGTACGATGA	26.1	38.1	52.6	55.0	1652 bp	1.0	40.1