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petA-psbJ

LSC · 62463-63428 · 966 bp

Back to Pugionium

Pi 0.00276

MarkerSeek score 48.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 4
pi: 0.00276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.0	58.1	1160-1161 bp	1.0	71.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.0	57.8	1161-1162 bp	1.0	70.5
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	1157-1158 bp	1.0	70.1
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.2	57.8	1158-1159 bp	1.0	69.4
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1155-1156 bp	1.0	68.7