Hotspot

ndhC-trnV

LSC · 50109-50978 · 870 bp

Back to Pugionium

Pi 0.00307

MarkerSeek score 48.20

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 4
pi: 0.00307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TTTCTCTGGCCCCTTCCTAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1011 bp	1.0	74.4
ndhC-trnV_p2	TCTCTGGCCCCTTCCTAATT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	1009 bp	1.0	72.6
ndhC-trnV_p3	TTCTCTGGCCCCTTCCTAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	1010 bp	1.0	72.6
ndhC-trnV_p4	TTTCTCTGGCCCCTTCCTAA	TTCGAGTCCGTATAGCCCTA	50.0	50.0	57.9	57.1	1010 bp	1.0	72.4
ndhC-trnV_p5	CTCTGGCCCCTTCCTAATTG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	57.6	58.7	1008 bp	1.0	72.0