Hotspot

rpoC2

LSC · 41191-45375 · 4185 bp

Back to Pueraria

Pi 0.00405

MarkerSeek score 35.50

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4185
variable_sites: 42
pi: 0.00405
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CGGACCACCATCAATCCATT	TCGCCAAATAAGGGTTAGCC	50.0	50.0	57.9	58.0	634 bp	1.0	82.8
rpoC2_p2	CGGACCACCATCAATCCATT	TCGTTAGCTCAGCACCAATC	50.0	50.0	57.9	58.0	886 bp	1.0	82.2
rpoC2_p3	CCGATACAGTGCCATGTTGA	TCGTTAGCTCAGCACCAATC	50.0	50.0	58.0	58.0	1388 bp	1.0	81.6
rpoC2_p4	CCGATACAGTGCCATGTTGA	TCGCCAAATAAGGGTTAGCC	50.0	50.0	58.0	58.0	1136 bp	1.0	81.6
rpoC2_p5	CCGATACAGTGCCATGTTGA	ATCCAATTAGTTCACCGGGC	50.0	50.0	58.0	57.9	1559 bp	1.0	81.1