Hotspot

rpl16

LSC · 80946-82575 · 1630 bp

Back to Pueraria

Pi 0.00477

MarkerSeek score 41.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1630
variable_sites: 19
pi: 0.00477
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGACCTCTTTCCTTTGGTTCT	TCATTTCATATTGATAGTATTGCT	42.9	25.0	56.5	52.1	1620-1771 bp	1.0	44.2
rpl16_p2	AAGACCTCTTTCCTTTGGTTCT	TCATTTCATATTGATAGTATTGCT	40.9	25.0	57.2	52.1	1621-1772 bp	1.0	44.2
rpl16_p3	AGACCTCTTTCCTTTGGTTC	TCATTTCATATTGATAGTATTGCT	45.0	25.0	54.9	52.1	1620-1771 bp	1.0	44.2
rpl16_p4	GACCTCTTTCCTTTGGTTCT	TCATTTCATATTGATAGTATTGCT	45.0	25.0	54.9	52.1	1619-1770 bp	1.0	44.2
rpl16_p5	AAAGACCTCTTTCCTTTGGTTCT	TCATTTCATATTGATAGTATTGCT	39.1	25.0	57.9	52.1	1622-1773 bp	1.0	44.2