Hotspot

psbE-petL

LSC · 64103-65046 · 944 bp

Back to Pueraria

Pi 0.00772

MarkerSeek score 40.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 18
pi: 0.00772
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1781-1798 bp	1.0	82.3
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1780-1797 bp	1.0	82.3
psbE-petL_p3	TGGTCATTGGAGCCTCCTAA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1831-1848 bp	1.0	82.0
psbE-petL_p4	AAAGGATCAAAACGGCCAGT	ACTGATCACCTCGTCGGTAT	45.0	50.0	58.0	57.9	1519-1560 bp	1.0	81.1
psbE-petL_p5	AAGGATCAAAACGGCCAGTT	ACTGATCACCTCGTCGGTAT	45.0	50.0	58.0	57.9	1518-1559 bp	1.0	81.1