Hotspot

psbK-trnQ-UUG

LSC · 54930-55404 · 475 bp

Back to Pueraria

Pi 0.00882

MarkerSeek score 50.30

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 10
pi: 0.00882
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	GGATTCAAGAAAGCGTAGGC	GATATGCTCTGGGACGGAAG	50.0	55.0	56.9	57.6	635-661 bp	1.0	71.8
psbK-trnQ-UUG_p2	TAGGCTTCGGGCAATTTCG	GATATGCTCTGGGACGGAAG	52.6	55.0	58.2	57.6	620-646 bp	1.0	71.0
psbK-trnQ-UUG_p3	GGATTCAAGAAAGCGTAGGC	GACCAAAACCCGTTGCCTTA	50.0	50.0	56.9	58.7	593-619 bp	1.0	70.2
psbK-trnQ-UUG_p4	TAGGCTTCGGGCAATTTCG	GACCAAAACCCGTTGCCTTA	52.6	50.0	58.2	58.7	578-604 bp	1.0	69.5
psbK-trnQ-UUG_p5	GGATTCAAGAAAGCGTAGGC	AATGGATATGCTCTGGGACG	50.0	50.0	56.9	57.2	639-665 bp	1.0	68.6